2023 ISAKOS Biennial Congress ePoster
Leukocyte-Poor PRP Suppresses the Progression of Post-Traumatic Knee Osteoarthritis
Yasumasa Momoi , MD JAPAN
Yoshitomo Saita, MD, PhD, Tokyo JAPAN
Nanako Yamamoto, MD, Tokyo JAPAN
Ryosuke Nakajima, MD, Bunkyo-Ku, Tokyo JAPAN
Sayuri Uchino, MD, PhD, Tokyo JAPAN
Takanori Wakayama, MD, Tokyo JAPAN
Yohei Kobayashi, MD, PhD, Tokyo JAPAN
Masashi Nagao, MD, PhD, Tokyo JAPAN
Muneaki Ishijima, MD, PhD, Tokyo JAPAN
Juntendo University, Tokyo, Bunkyo-ku, JAPAN
FDA Status Not Applicable
Summary
Leukocyte-poor PRP suppresses the progression of post-traumatic knee osteoarthritis.
ePosters will be available shortly before Congress
Abstract
Platelet-rich plasma (PRP) is an emerging therapeutic strategy for treatment of osteoarthritis (OA); however, the benefit of including leukocytes in the PRP product remains controversial. Purpose: To compare the effect of Leukocyte-poor PRP (LP) and Leukocyte-rich PRP (LR) in a mouse knee OA model. Methods: A knee OA model (Destabilized Medial Meniscus: DMM) was induced in the right hind limbs of 10-week-old wild female mouse (C57BL / 6J). PBS (control group), LP and LR were intra-articular injected at 2, 4, and 6 weeks after surgery. (N=12 in each group). Weightbearing distribution of right hindlimb was measured on month 0, 1, 3 after surgery and the cartilage degeneration in medial femoral condyle stained with Safranin-O was scored by OARSI scoring after sacrificed on month 3. Results: Weightbearing distribution (%) on the affected side is pre (PBS group 45.2 ± 7. 4, LP group: 48.2 ± 8.4, LR group: 44.6 ± 9.7, P = 0.55), 1M (PBS group 45.0 ± 9.9, LP group: 47.3 ± 8.0, LR group: 44.8 ± 10.2, P = 0.77), 3M (PBS group 53.3 ± 8.4, LP group: 52.3 ± 6.8, LR group: 52.1 ± 6.2, P = 0.92), and no significant difference was observed in each group. In OARSI scoring, PBS group: 4.4 ± 1.2, LP group: 2.5 ± 1.2, LR group: 2.9 ± 2.0, which significantly suppressed cartilage destruction in LP group compared with PBS group (p = 0.01), but there were no significant difference between the LP and LR groups. Discussions and Conclusion: In the behavioral test, there were no significant difference in each group. It was considered that the DMM model had a relatively slow progression of OA. Histological evaluation showed the effect of suppressing cartilage degeneration in only LP, which may demonstrate that Leukocyte-poor PRP suppress the progression of post-traumatic knee osteoarthritis more than Leukocyte-rich PRP.