2015 ISAKOS Biennial Congress ePoster #1247

Histological Analysys of Hamstring Tendons After Decontamination

Thiago R. Protta, MD, Sao Paulo, Sao Paulo BRAZIL
Marcelo Schmidt Navarro, MD, Sao Caetano Do Sul, Sao Paulo BRAZIL
Adriana Vanzin, MD, Sao Paulo, sao paulo BRAZIL
Gabriel Cunha Saheb, Santo André, Sao Paulo BRAZIL
Ana Maria Mader, MD, Sao Paulo, sao paulo BRAZIL
Leonardo Kiman Lee, MD, Sao Paulo, sp BRAZIL
Karise Alves, Sao Paulo, sao paulo BRAZIL

university abc, sao paulo, sao paulo, BRAZIL

FDA Status Not Applicable

Summary: HISTOLOGICAL ANALYSYS OF HAMSTRING TENDONS AFTER DECONTAMINATION

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Abstract:

Introduction

Hamstring tendons are often used during many orthopedics surgeries. The risk of contamination is low but always present. Some solutions showed great results to decontaminate the tendons but the effect of these solutions in the grafts motivates research regarding cell viability.

Purpose

Evaluate cell viability from tendon grafts after decomtamination using chlorhexidine and povidone – iodine 10%.

Materials And Methods

Remaining part from grafts removed during 21 procedures from ACL reconstruction were cut and divided into 5 groups (105 samples). Group 1: was the control; Group 2 and 3 we used povidone iodine during 2 and 5 minutes respectively; Group 4 and 5 we used chlorhexidine during 2 and 5 minutes respectively. After that, the grafts were preserved and sent to the pathology service and paraffin blocks were made followed by histological sections. The pathologist used visual parameters present in the vascular phase, and cell apoptosis semiquantitatively. For statistical analysis we used the method of Kruskal-Wallis and Bonferroni test with p <0.01.

Results

We observed significant difference between the control and the groups submitted to solutions for 5 minutes (both solutions) when the comparison was made between control and groups of two minutes groups no significant difference in autolysis was found.

Conclusion

The use of aseptic solutions (chlorhexidine and povidone-iodine) for a period of two minutes showed no significant difference when compared with the control group regarding autolysis.