2015 ISAKOS Biennial Congress ePoster #417
Rabbit Tendon-Bone Healing Using an Injectable Collagen Gel Combined with rhBMP-2
Kwang-Won Lee, MD, PhD, Daejeon KOREA, REPUBLIC OF
Kwang-Il Lee, PhD, Seoul KOREA, REPUBLIC OF
Jung-Soo Lee, PhD, Seoul KOREA, REPUBLIC OF
Yong-Han Cha, MD, Daejeon KOREA, REPUBLIC OF
Choon Myen Kim, MD, Daejeon KOREA, REPUBLIC OF
Dae Suk Yang, MD, Daejeon KOREA, REPUBLIC OF
Department of Orthopaedic Surgery, Eulji University Hospital, Daejeon, KOREA
FDA Status Not Applicable
Summary: Viscous collagen gel can be an effective carrier for rhBMP-2 delivery into surgical sites, and that the injectable rhBMP-2 containing collagen gel may be applied for the enhancement of tendon-bone interface healing in the future.
Abstract:
This study examines the hypothesis that the injectable collagen gel can be effective carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2)’s localization to the healing tendon-bone interface. In 36 mature New Zealand White rabbits, the upper long digital extensor tendon was cut and inserted into the proximal tibial bone tunnel. Then, a rhBMP-2 containing collagen gel was injected into the tendon–bone tunnel interface using syringe. Histological and biomechanical assessments of the tendon–bone interface were conducted at 3 and 6 weeks after implantation.
In vitro testing showed that the semi-viscous collagen gel at room temperature was transformed into a firm gel state at 37?. The rhBMP-2 release profile showed that rhBMP-2 was released from the collagen gel for more than 28 days. In vivo testing showed that fibrocartilage and new bone are formed at the interface at 6 weeks after injection of rhBMP-2. On radiography, spotty calcification appeared and enthesis-like tissue was produced successfully in the tendon at 6 weeks after injection of rhBMP-2.
Use of the viscous collagen gel and rhBMP-2 mixture increased the fusion rate between the bone tunnel and tissue graft. This study demonstrates that viscous collagen gel can be an effective carrier for rhBMP-2 delivery into surgical sites, and that the injectable rhBMP-2 containing collagen gel may be applied for the enhancement of tendon-bone interface healing in the future.