2015 ISAKOS Biennial Congress ePoster #419

Role of PRP and Hyaluronic Acid in the Regeneration of Osteochondral Lesions. An Animal Model

David H. Figueroa, MD, Santiago, RM CHILE
Rafael Martinez, MD, PhD, Santiago, Region Metropolitana CHILE
Rafael Calvo, MD, Santiago CHILE
Francisco Figueroa, MD, Santiago, RM CHILE
Ximena Ahumada, MD, Santiago, Region Metropolitana CHILE
Marcela Gallegos, MD, Santiago CHILE
Paul Arias, MD, Santiago, Region Metropolitana CHILE
Facultad de Medicina Clinica Alemana - Universidad del Desarrollo, Santiago, Region Metropolitana, CHILE

FDA Status Not Applicable

Summary: Aim: To compare the effect of Platelet rich plasma (PRP) and Hyaluronic acid (HA) in the regeneration of osteochondral lesions (OCL) in mice. Conclusion: PRP group generated better regeneration of OCL compared with HA group and the control group.

Abstract:

Aim

To compare the effect of Platelet rich plasma (PRP) and Hyaluronic acid (HA) in the regeneration of osteochondral lesions (OCL) in mice.

Methods

This controlled laboratory study was approved by the research ethics board at our institution. OCL were created in the trochlear groove in 24 adult (15 weeks old) BKS strain mice. This animal model was previously validated and published by our group. 8 animals (16 knees) were randomly assigned to receive normal saline (control group), PRP (PRP group) or HA (HA group) at the end of the surgery. At 7 days the animals were sacrificed and the knees harvested. Regeneration tissue was blinded evaluated by hematoxylin-eosin and safranin-O stains and using the International Cartilage Repair Society (ICRS-II) scoring scale. Statistical analysis: average and standard deviation were evaluated using unpaired Student t tests

Significance

defined as p<0.05).

Results

In the evaluation at 7 days the hematoxylin-eosin and safranin-O stains demonstrated better fill of the lesions and more normal appearing than control group and HA group. In the ICRS-II scoring scale evaluation the PRP group has significantly higher average (15.5+2.5) than control group (9.7+0.8) and HA group (5.2+1.3). The differences between the control group and HA were also statistically significant.

Conclusion

In our animal study the PRP group generated better regeneration of OCL compared with HA group and the control group. Interestingly the HA group had worse results compared with the control group. This higher potential for healing in the PRP group reveals that application of PRP in the treatment of OCL could be beneficial inducing chondral reparation