2017 ISAKOS Biennial Congress ePoster #318

 

Bone/Cartilage Formation In 3D Printed Biomaterials Using Msc-Derived Cells

Seda Vatansever, Prof, Manisa TURKEY
Feyzan Ozdal Kurt, Ph.D., Manisa TURKEY
Remziye Kendirci, MPHil, Manisa TURKEY
Ersin AytaƧ, Dr, Nicosia CYPRUS
Gorkem Say, Dr, Nicosia CYPRUS
Emil Mammadov, Assistant Prof, Nicosia CYPRUS

Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey, Near East University, Experimental Health Science Research Center, Nicosia, North Cyprus, Manisa, TURKEY

FDA Status Not Applicable

Summary

3D PLA biomatrix were support bone or cartilage formation, therefore, it may use with cell in vivo experimental studies.

ePosters will be available shortly before Congress

Abstract

In the cell therapy principles, because of potential source of stem cells which derived from different sources can be used for research topics as a drug. When the stem cells could be used for therapeutic purposes, transferring of these cells into in vivo condition, biocompatible materials can be use to ensure their compliance. New developments in the tissue engineering, 3D printing method has experienced a rapid entry into production of biocompatible and biodegradable materials.
In this study, we aimed that the formation of both bone and cartilage from mesenchymal stem cells (MSC) derived osteoblast or chondroblast cells on 3D printed biomaterials with polylactic acid (PLA) polymers.
Biomatrix for the production of PLA polymers to be used and the PLA filament diameter of 1.75 mm, three roller bearings assistance requested by the printer from the filament was strapped using SolidWorks 3D printer. Mesenchymal stem cells was collected from rat bone marrow and cultured for 2 weeks for proliferation in a-MEM medium containing 10% fetal bovine serum, 1% L-glutamine, 1% penicillin-streptomycin, 1% gentamicine and 2mM Amphotericin B. They were then cultured in either osteogenic or chondrogenic differentiation using standart kits according their protocols for 2 weeks. The characterization of cells were analysed using Alizarin Red and Alcian blue staining for their routine protocol. Distribution of anti-osteonectin and anti-chondronectin were also analysed by immunohistochemically. Differentiated 1x106 cells / cm3 (osteoblast or chondroblast) was added in the 3D biomatrix and cultured 2 weeks. Both histochemical (Alizarin Red and Alcian Blue) and immunohistochemical (anti-osteonectin and anti-chondronectin) staining were performed after fixation with 4% paraformaldeyhde.
Osteogenic and chondrogenic differentiation from MSC were performed after 2 weeks culture condition. They were positively stained with hictochemically and immunohistochemically. When transferred the cells onto 3D PLA biomatrix, their differentiation were continue and bone or cartilage formation were observed after histochemical analyses.