2017 ISAKOS Biennial Congress ePoster #302
Metabolomic Profiling of Serum and Capsular Tissue from Patients with Adhesive Capsulitis: A Preliminary Study
Hyo-Jin Lee, MD, Prof., Seoul KOREA, REPUBLIC OF
Yang-Soo Kim, MD, PhD, Prof., Seoul KOREA, REPUBLIC OF
In Park, MD, Seoul KOREA, REPUBLIC OF
Hong-Ki Jin, MD, Busan KOREA, REPUBLIC OF
Dong-Hyuk Sun, MD, Seoul, Seoul KOREA, REPUBLIC OF
Sung-Ryeoll Park, MD, Seoul KOREA, REPUBLIC OF
Jin Hong Kim, MD, Seoul KOREA, REPUBLIC OF
Dongjin Kim, MD, Seoul KOREA, REPUBLIC OF
Jong-Ho Kim, MD, Seoul KOREA, REPUBLIC OF
Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, KOREA, REPUBLIC OF
FDA Status Cleared
Summary
Levels of sphingomyelin and glycerophospholipid were increased in anterior capsule and rotator interval tissue of adhesive capsulitis. These metabolites showed a positive correlation with serum total cholesterol level. Regarding the results of the study, control of serum level of total cholesterol may have positive effect on the treatment of adhesive capsulitis.
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Abstract
Introduction
Adhesive capsulitis of the shoulder is a common disease that debilitates normal life, but its etiology is unclear. Using mass spectrometry, we evaluated the metabolic profiles of samples from patients with rotator cuff tears with adhesive capsulitis, or primary adhesive capsulitis, and compared these with samples from a control group.
Methods
A total of 28 patients consisting of 10 patients with rotator cuff tears with adhesive capsulitis (Group I), nine patients with primary adhesive capsulitis (Group II), and nine controls were enrolled. Blood (serum) and tissue from the rotator interval and anterior capsule were collected from all patients. In all, 82 samples were analyzed for metabolite profiling using the AbsoluteIDQTMp180 Kit. Amino acids and biogenic amines were analyzed quantitatively by stable isotope dilution in LC-MS/MS using an API 4000Qtrap (ABSCIEX, Foster City, CA, USA) mass spectrometer. For acylcarnitines, hexoses, phosphor-and sphingolipids, mass spectrometry analysis was done by the FIA-MS/MS method.
Results
Comparison of the 186 metabolites evaluated among groups revealed that groups I and II had a significantly higher concentration of sphingolipids (SM C24:1 in serum, SM C22:3 in rotator interval) than the control group. The concentration of glycerophospholipid (PC aa C30:0) was significantly higher in the anterior capsule tissue of groups I and II than that of the control group. Total cholesterol level was positively correlated with sphingolipid concentration in serum and rotator interval tissue. There were no significant differences in the 186 metabolites evaluated between groups I and II.
Conclusion
Metabolic profiling showed that levels of lipid-related metabolites (sphingomyelin and glycerophospholipid) were increased in the anterior capsule tissue and rotator interval tissue of patients with adhesive capsulitis. Furthermore, these metabolites had positive correlation with serum level of total cholesterol. Regarding the results indicating that serum level of total cholesterol has a significant association with the pathogenesis of adhesive capsulitis, control of serum level of total cholesterol may have positive effect on the treatment of adhesive capsulitis.