Summary

This study provided the experimental evidence that in knee OA rats model, FD-PRP injection exhibited a histologically and behaviorally significant improvement. In terms of the timing to manufacture the FD-PRP, no inflammation group was significantly higher concentration of PDGF-BB having a capability of a chondrogenic bioreactivity.

Abstract

Introduction

Recently, platelet rich plasma (PRP) has been extensively used treatment of knee osteoarthritis (OA) and consequently, a variable methods and techniques to manufacture a PRP have emerged in clinical practice.
A freeze-dried PRP(FD-PRP) is reported to preserve a bioreactivity of growth factors for a semi-permanent, having a capability of selectively the timing to correct a peripheral blood. However, it is unclear which the timing to collect a peripheral blood from OA patients would be better characteristic to manufacture a higher concentration of growth factor of freeze-dried PRP with or without inflammatory on an intraarticular of its themselves. The aim of this study was to assess the efficacy of intra-articular FD-PRP injection in knee osteoarthritis and to determine the timing to collect a peripheral blood.

Methods

To prepare the two types of allografted FD-PRP, whole blood was collected from both rats healthy or knee arthritis with intra-articular injections of monosodium iodoacetate (MIA). Thereafter, FD-PRP were prepared by second spinning and the using CaCl2: the Healthy rats-derived FD-PRP(HD-FD-PRP) and the Arthritis rats-derived FD-PRP(AD-FD-PRP).
Knee osteoarthritis was induced in male Sprague–Dawley rats with intra-articular injections of monosodium iodoacetate (MIA) on day 0. On day 7, PRP was injected into the right knee of rats and saline was injected into the left knee as a control. We measured the struggle threshold of knee extension angle in order to assess pain of the knee joint on day 7,14, and 21. Rats were euthanized at day 21 for histological assessment of synovial tissue and cartilage. Concentrations of growth factors in the injected PRP were assessed to determine their association with outcomes.

Results

In both PRP groups significantly reduced pain responses throughout 2 weeks post-dosing.
In terms of a quantitive histological assessment, structural changes in the synovial tissue and cartilage degeneration were significantly inhibited in both groups compared with the controls. Additionally, the HD-FD-PRP yielded favorable results than the AD-FD-PRP. The concentrations of PDGF-BB were significantly higher, VEGF was significantly lower in HD-FD-PRP.

Conclusions

This study provided the experimental evidence that in knee OA rats model, FD-PRP injection exhibited a histologically and behaviorally significant improvement. In terms of the timing to manufacture the FD-PRP, no inflammation group was significantly higher concentration of PDGF-BB having a capability of a chondrogenic bioreactivity. It is suggested that FD-PRP might have the potential to have an efficacy on a mild OA.