2023 ISAKOS Biennial Congress ePoster
Evaluation Of Different Commercial Hyaluronic Acids As A Vehicle for Injection of Human Adipose-Derived Mesenchymal Stem Cells
José Ricardo Dantas Moura Costa, MD, São Paulo, São Paulo BRAZIL
Nathalia Bofill Burger, Uruguaiana, Rio Grande do Sul BRAZIL
Marcel Henrique Arcuri, Md, Sao Paulo BRAZIL
Diego Costa Astur, MD, PhD, São Paulo, SP BRAZIL
Camila Cohen Kaleka, PhD, São Paulo, SP BRAZIL
Pedro Debieux, MD, PhD, São Paulo, SP BRAZIL
Moises Cohen, MD, PhD, Prof., São Paulo, SP BRAZIL
Hospital Israelita Albert Einstein, São Paulo, São Paulo, SP, BRAZIL
FDA Status Not Applicable
Summary
After valuation of different commercial hyaluronic acids, ehese data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs.
ePosters will be available shortly before Congress
Abstract
Objective
The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).
Methods
AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).
Results
The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.
Conclusion
These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.