2025 ISAKOS Biennial Congress ePoster
Synthetic Polydioxanone-Based Scaffold For Cartilage Repair In A Sheep Model
Pedro Debieux, MD, PhD, São Paulo, SP BRAZIL
Moises Cohen, MD, PhD, Prof., São Paulo, SP BRAZIL
Camila Cohen Kaleka, PhD, São Paulo, SP BRAZIL
Emanuel Vitor Pereira Apolonio, DVM MS, Botucatu, São Paulo BRAZIL
Vittoria Guerra Altheman, Msc, Botucatu, São paulo BRAZIL
Gustavo Guadalupe, MD, São Paulo BRAZIL
Ana Liz Garcia Alves, PhD, Botucatu, SAO PAULO BRAZIL
São Paulo State University (UNESP) / Hospital Israelita Albert Einstein, São Paulo, SP, BRAZIL
FDA Status Not Applicable
Summary
This study evaluated the effectiveness of a polydioxanone (PDO) scaffold for articular cartilage repair in sheep, finding that it provides better cell support and chondrogenic differentiation compared to microfracture alone and a collagen membrane, with no adverse reactions observed.
ePosters will be available shortly before Congress
Abstract
Purpose
Despite improving therapeutic options, satisfactory cartilage repair
remains a challenging joint condition in human and animal orthopedics. This
study aimed to verify the potential of a synthetic scaffold based on
polydioxanone (PDO) in articular cartilage repair compared to a commercially
available porcine collagen (type I/III) membrane and microfracture alone in a
study model in sheep. Material & Methods: Chondral defects 10 mm in diameter
were created in the weight-bearing center of the medial femoral condyle in both
limbs of 16 adult Ile de France sheep. The animals were divided into four
groups: (1) microfracture only, (2) microfracture with type I/III collagen
membrane, (3) microfracture with PDO scaffold, and (4) control group without
treatment. The quality of cartilage repair was assessed macroscopically after 26
weeks in a masked study by three experts using the ICRS score (ICC: 89.8%),
and the synovial concentration of cartilage biomarkers such as hyaluronan,
CS846, CP-II, and C2C was measured by ELISA assay. Results: No adverse
reactions were observable at the sheep joint using the PDO scaffold. Both
polydioxanone and collagen scaffolds presented superior ICRS scores
compared to the control group (P ≤ 0,0001 and P ≤ 0,01, respectively).
Cartilage repair in the PDO scaffold group was better than in the microfractureonly
group (P ≤ 0.01), suggesting that the polydioxanone scaffold provides
better cell support and promotes chondrogenic differentiation of local
mesenchymal cells. In addition, there were no statistical differences between
groups in sheep synovial fluid biomarkers. Conclusion: The study results show
that polydioxanone membrane is a safe scaffold that provides better cell support
and shows potential improvement in chondrogenic differentiation, which may
improve the quality of cartilage repair. In addition, this PDO membrane's
mechanical and physicochemical properties allow personalization and fixation
by suture on the host of.