2025 ISAKOS Biennial Congress ePoster
Microfibrous Scaffolds with Dual-Triphase Structural and Compositional Configurations Reshaping the Lipidomic Profile for Osteotendinous Junction Healing of a Rotator Cuff Model
Lang Bai, MD, Xi'an, Shaanxi CHINA
Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, CHINA
FDA Status Not Applicable
Summary
This study introduces electrohydrodynamic printed scaffolds, integrating polycaprolactone with bioactive compounds for rotator cuff repair, demonstrating enhanced cellular viability, differentiation, biomechanical strength, and lipidomic modulation, offering a promising strategy for improving clinical outcomes.
ePosters will be available shortly before Congress
Abstract
Rotator cuff injuries can lead to chronic pain and limited shoulder function due to retears and scar formation at the osteotendinous junction (OTJ). To combat this, our study employed electrohydrodynamic printed dual-triphase microfibrous scaffolds (TS+TC group), targeting the critical phases of OTJ repair. The scaffolds, composed of polycaprolactone with nano-hydroxyapatite, nano-magnesium oxide, and kartogenin, showed over 95% stem cell viability, with enhanced expression of tenomodulin, SRY-Box transcription factor 9, and runt-related Transcription factor 2. In the TS+TC group, tendon markers tenomodulin and scleraxis increased by 7.3 and 5.7-fold, and chondrocyte and osteoblast markers also showed significant upregulation.
In vivo, the TS+TC scaffolds led to superior healing, with biomechanical testing indicating a maximum failure load of 27.0 ± 4.2 N and ultimate stress of 5.5 ± 1.0 MPa at 6 weeks, and even greater improvements at 12 weeks. Lipidomic analysis revealed a substantial upregulation of phosphatidylcholine, phosphatidylinositol, cardiolipin, and phosphatidylserine in the suture-only group, which was then downregulated after scaffold implantation, emphasizing the scaffolds' role in normalizing lipid profiles to enhance repair and recovery. These findings demonstrate the scaffolds' effectiveness in promoting tissue regeneration and improving clinical outcomes for rotator cuff injuries.
This study highlights the potential of dual-triphase microfibrous scaffolds in enhancing rotator cuff enthesis repair by supporting cellular differentiation, improving biomechanical properties, modulating the lipidomic environment, and promoting tissue regeneration. These findings suggest a promising approach for clinical strategies in treating rotator cuff injuries.