2025 ISAKOS Biennial Congress ePoster
Injectable ChitHCl-DDA tissue adhesive with high adhesive strength and biocompatibility for torn meniscus repair and regeneration
Jia-Lin Wu, MD, MS, Taipei TAIWAN
Pei-Chun Wong, PhD, Taipei, Please Select TAIWAN
Taipei Medical University/Taipei Medical University Hospital , Taipei , TAIWAN
FDA Status Not Applicable
Summary
A novel injectable tissue adhesive developed from chitosan hydrochloride and oxidative periodate-oxidized dextran, combined with a chitosan-based hydrogel and oxidative dextran shows promising potential for enhancing meniscus tissue repair and regeneration.
ePosters will be available shortly before Congress
Abstract
Meniscus repair surgery often faces challenges related to suture pull-through due to the sharp edges of traditional sutures. To address this issue, we explored a novel tissue adhesive developed from chitosan hydrochloride and oxidative periodate-oxidized dextran (ChitHCl-DDA), combined with a chitosan-based hydrogel and oxidative dextran, as an alternative for meniscus repair. Comprehensive evaluations were conducted to characterize the adhesive properties, including viscoelasticity, viscosity, lap shear stress, Fourier transform infrared (FTIR) spectroscopy, swelling ratio, and degradation behavior. To assess biological compatibility, we performed MTT assays, alcian blue staining, migration assays, cell behavior observations, and protein expression analyses to evaluate cell viability and response. Further, ex vivo and in vivo tests were conducted to examine tissue regeneration and biocompatibility. The results demonstrated that ChitHCl-DDA exhibited adhesive solid properties, high cell viability, and favorable cellular responses. This tissue adhesive shows promising potential for enhancing meniscus tissue repair and regeneration.