Fresh osteochondral allograft transplantation (FOCA) is a valid technique for treating osteochondral lesions in the knee. The viability of chondrocytes and the integration of the graft are key factors for the success of this procedure. The use of advanced imaging techniques, such as MRI Mapping, allows for a non-invasive evaluation of cartilage integrity. However, the viability of the transplant can be analyzed through immunohistochemical methods via biopsy.

Objectives: To evaluate the viability of chondrocytes and the integration of fresh osteochondral allografts through histological studies, and to correlate these findings with the images obtained from MRI mapping.

Methods

A total of 10 patients diagnosed with osteochondral lesions in the knee femoral condyles diagnosed by MRI were included in this study. They all underwent FOCA procedure. Clinical follow up was performed using functional scales every 3 months (KSS, modified Merlé d’Aubigné, KOOS) and X-rays at sixth and twelfth months post-surgery. One year after surgery, a MRI with cartilage mapping and a biopsy of the allograft was performed. The viability of the implanted chondrocytes was evaluated with confocal fluorescence microscopy. For quantitative T2 mapping, regions of interest were drawn in the deep and superficial layers of allograft and control cartilage.

Results

Histological analyses showed a high viability of chondrocytes in the majority of cases, above 72% (p ≤ 0.05). The MRI Mapping accurately identified areas of cartilage integrity and degeneration, showing a significant correlation with histological results. There was no difference between the mean T2 values in the deep zone of the allograft and control cartilage at one year (p < 0.01), but mean T2 values were higher in the superficial zone of the allograft cartilage (p < 0.05). There was a significant improvement in functional outcomes in all patients. No correlation was found between functional and radiological outcomes.

Conclusions

Fresh osteochondral allograft transplantation in knee femoral condyles proves to be a viable technique, with a high chondrocyte viability rate and good graft integration, with positive clinical and functional results one year after implantation. The MRI mapping is a valuable tool for non-invasive evaluation of cartilage integrity and transplant viability. Graft integration was complete in all patients.

Matrix-Induced Autologous Chondrocyte Implantation in Adolescents - 10 Year Results From a Propensity Score Matched Cohort Study ePoster Presentation Johannes Weishorn, MD, GERMANY Johanna Wiegand, MD, GERMANY Severin Zietzschmann, MD, GERMANY Raphael Trefzer, MD, GERMANY Tilman Walker, GERMANY Kevin-Arno Koch, MD, GERMANY
Evaluating The Role Of Spect/Ct For The Detection Of Cartilage Lesions In The Knee: A Systematic Review ePoster Presentation Larissa Rix, MBioMedSci, UNITED KINGDOM Samuel Tushingham, Phd, UNITED KINGDOM Karina Wright, PhD, UNITED KINGDOM Martyn Snow, FRCS, UNITED KINGDOM
Artificial Collagen Membrane Fixed By All-Suture Device Can Be Better Clinical Outcomes In Autologous Chondrocyte Implantation For Middle-Aged And Elderly Patients ePoster Presentation Akira Kawai, MD, JAPAN Tomoya Iseki, MD, PhD, JAPAN Ryo Kanto, MD, PhD., JAPAN Yoshitaka Nakao, MD, JAPAN Shunsuke Akai, MD, JAPAN Hiroshi Nakayama, MD., PhD., JAPAN Toshiya Tachibana, MD, PhD, JAPAN
Patient-Specific Guides For Subchondroplasty Of The Knee: Short-Term Clinical Outcomes ePoster Presentation Andrea Pratobevera, MD, ITALY Marcello Motta, MD, ITALY Giulio Vittone, MD, ITALY Maristella Francesca Saccomanno, MD, PhD, ITALY Sofia Ravo, MD, ITALY Giuseppe Milano, Prof., ITALY
Promising Long Term Functional Results For Autologous Osteochondral Transplantation (Oats) ePoster Presentation Jorge Simon Isla, MD, CHILE David H. Figueroa, MD, CHILE Minerva Gabriela Itriago, MD, CHILE Francisco Figueroa, MD, CHILE
Transosseous Wrapping Technique Using Resorbable Suture Tape For Fixation Of Osteochondral Lesions In The Knee Is A Safe Procedure Without Complications. ePoster Presentation Martin Rathcke, MD, DENMARK Lars Konradsen, MD, DENMARK
No Difference In Outcomes Between Coblation And Mechanical Chondroplasty In Patients Undergoing Autologous Chondrocyte Implantation (Aci) ePoster Presentation Eric Milliron, BS, UNITED STATES Connor Jacob, BS, UNITED STATES Parker Cavendish, BS, UNITED STATES Tyler Barker, PhD, UNITED STATES Jelle P. van der List, MD, PhD, NETHERLANDS Cory Meixner, MD, UNITED STATES Christopher C. Kaeding, MD, UNITED STATES Robert A. Magnussen, MD, MPH, UNITED STATES David C. Flanigan, MD, UNITED STATES
Biomimetic Scaffold for the Treatment of Chondral and Osteochondral Lesions of the Knee: Clinical Results at Long Term Follow-up. ePoster Presentation Iacopo Romandini, MD, QATAR Alessandro Di Martino, MD, ITALY Luca Andriolo, MD, ITALY Davide Reale, MD, ITALY Luca Solaro, MD, ITALY Marco Franceschini, MD, ITALY Stefano Zaffagnini, MD, Prof., ITALY Giuseppe Filardo, MD, PhD, MBA, Prof., SWITZERLAND
Correlation Between MRI And Histological Analyses For Repair Tissue Of Knee Chondral Lesions Treated With Allogenic Mesenchymal Stem Cell-Based Therapy Or Microfracture In A Phase III Clinical Trial Cohort ePoster Presentation Kazunori Shimomura, MD, PhD, Prof., JAPAN Atsuya Watanabe, MD, PhD, JAPAN Akira Tsujii, MD, PhD, JAPAN Kazutaka Kinugasa, MD, PhD, JAPAN Ryohei Uchida, MD, PhD, JAPAN Yasukazu Yonetani, MD, PhD, JAPAN Yoshinari Tanaka, MD, PhD, JAPAN Yukiyoshi Toritsuka, MD, PhD, JAPAN Norimasa Nakamura, MD, PhD, JAPAN
Pullout Strength Of Single Verses Multiple Synthetic Chondral Implants ePoster Presentation David C. Flanigan, MD, UNITED STATES Emma Flanigan, research student, UNITED STATES Emmy Aram, research student, UNITED STATES Nathan Castro, PhD, UNITED STATES Luke Aram, PhD, UNITED STATES
New Treatment Algorithm for Unstable, Unsalvageable Osteochondritis Dissecans of Knee ePoster Presentation Kukuh Dwiputra Hernugrahanto, MD, INDONESIA Jifaldi Afrian Maharaja Dinda Sedar, MD, INDONESIA Dwikora Novembri Utomo, MD, PhD, INDONESIA Nicolaas C. Budhiparama, MD, PhD (LUMC, NL), PhD (UGM, ID), FICS, Prof., INDONESIA
Return To Play And Continued Participation In Elite Sport After Microfracture For Chondral Lesions In The Knee – A Matched Controlled Cohort Study ePoster Presentation David Johannes Haslhofer, MD, AUSTRIA Jobe Shatrov, MD, AUSTRALIA Mary Jones, MSc, Grad. Dip. Phys., UNITED KINGDOM Wahid Abdul, BSc(Hons), MBBCh, MRCS, FRCS(Tr&Orth), MSc(SEM), UNITED KINGDOM Arman Motesharei, PhD, UNITED KINGDOM Simon Ball, MA, FRCS(Tr&Orth), UNITED KINGDOM Andy Williams, MBBS, FRCS(Orth), FFSEM(UK), UNITED KINGDOM
Fresh allograft transplantation in patients with large femoral osteochondral defects – A case series of a single center study ePoster Presentation Joao Pedro Oliveira, MD, PORTUGAL João Castro Mendes, MD, PORTUGAL Fernando Judas, PhD, PORTUGAL Fernando Fonseca, MD, PhD, PORTUGAL
Preoperative Cartilage Damage And Varus Deformity Of Tibia Are Factors Affecting Cartilage Status Improvement In The Medial Compartment After Open Wedge High Tibial Osteotomy ePoster Presentation Takashi Hoshino, MD, PhD, JAPAN Tomomasa Nakamura, MD, PhD, JAPAN Yusuke Nakagawa, JAPAN Mai Katakura, MD, PhD, JAPAN Ichiro Sekiya, MD, PhD, JAPAN Hideyuki Koga, Prof., MD, PhD, JAPAN
Fixation Of Pure Chondral Injuries Of The Knee: Systematic Review ePoster Presentation Fernando Martin, MD, CHILE Javier Ignacio González, MD, CHILE Maximiliano Espinosa, MD, CHILE

Menu

Search Filters

Viability Of Chondrocytes And Radiological Evaluation Using Mri Mapping Of The Cartilage In Fresh Osteochondral Allograft Transplants In Knee Femoral Condyles.

Viability Of Chondrocytes And Radiological Evaluation Using Mri Mapping Of The Cartilage In Fresh Osteochondral Allograft Transplants In Knee Femoral Condyles.

Introduction

Methods

Results

Conclusions

Knee Cartilage ePosters

Sign-in to ISAKOS

Viability Of Chondrocytes And Radiological Evaluation Using Mri Mapping Of The Cartilage In Fresh Osteochondral Allograft Transplants In Knee Femoral Condyles.

Viability Of Chondrocytes And Radiological Evaluation Using Mri Mapping Of The Cartilage In Fresh Osteochondral Allograft Transplants In Knee Femoral Condyles.

Introduction

Methods

Results

Conclusions