Due to the limited resources of fresh cartilage allograft and the extended time needed for serologic and microbiologic testing and donor-recipient matching, an improved cartilage preservation technique is in a great need to further extend the implementation of the osteochondral allograft transplantation. Vitrification, a process that transfers a liquid solution into a glassy solid without ice formation, is a promising technique for tissue cryopreservation. The authors hypothesize that the combination of nanowarming with ice-free cryopreservation by vitrification will help scale up articular cartilage cryopreservation to larger specimen sizes while improving cell viability and maintaining cartilage matrix properties compared with conventional vitrified tissue warming using large articular cartilage specimens.

Methods

Femoral weight bearing condyles of sexually mature domestic Yorkshire cross pigs were obtained and no animals were sacrificed specifically for this study. Osteochondral pieces of the femoral condyle were harvested and separated into three groups: fresh, convection and nanowarming. The osteochondral pieces in the fresh group were kept in a Dulbecco’s minimum essential media while convection and nanowarming specimens were both vitrified ± 2 mg/mL Fe nanoparticles. The vitrified tissues were then cooled and rewarmed using either a two stage convective warming process while the nanowarming group was rewarmed in 80 seconds using a solid state induction power supply. Cell viability (using fluorescence live/dead staining with calcein AM and ethidium homodimer) and metabolic activity (using alamarBlue assay) were then assessed among the three groups.

Results

Fluorescence live/dead staining images revealed that an increased number of living cells with nanowarming cartilage compared to the convection warmed group. Cell metabolic activity increased in both the convection group and nanowarming groups; however significantly higher cell metabolic activity was found in the nanowarming group when compared to the convection group (p < 0.0001). No significant difference in conductivity was found between the fresh cartilage group, convection or the nanowarming group.

Discussion And Conclusion

The live/dead staining and cell activity results indicated that nanowarming outperformed the conventional convection warming method by rescuing more cells and maintaining cell activity for large cartilage cryopreservation. Nanowarming caused very limited alterations on the cartilage permeability with conductivity results as well. This study pioneers the application of nanowarming for large sized articular cartilage cryopreserved specimens. This biobanking method for cartilage preservation has the chance to improve the utilization of the limited supply of allografts, increase long term allograft survival in vivo, and extend the indications for osteochondral allograft transplantation.

Mechanical Evaluation of Commercially Available Fibrin Sealant Adhesives for Cartilage Repair Abstract Presentation 4 minutes Arya Amirhekmat, BA, UNITED STATES Wendy E. Brown, PhD, UNITED STATES Kyriacos Athanasiou, PhD, PE, UNITED STATES Dean Wang, MD, UNITED STATES
Acellular Stem Cell Derivatives for the Treatment of Muscle Injuries: Molecular Effect in an In Vivo Animal Study Abstract Presentation 6 minutes Alex Vaisman, MD, Prof., CHILE Rodrigo Guiloff, MD, Prof, CHILE Marcelo Ezquer, PhD, CHILE Sergio Arellano, MD, CHILE Iris Delgado, CHILE David H. Figueroa, MD, CHILE Paulette Conget, PhD, CHILE
Functional Outcomes Of Platelet Rich Fibrin Membrane With Arthroscopic Debridement In Chondral Defect Of Patella.: A Clinico Radiological Study. Abstract Presentation 5 minutes Amit Lakhani, MS, DNB, MRCPS(Glassg), MNAS, INDIA Dr Ena Sharma, MDS, INDIA
Biomechanical Comparison of a Novel, Multi-Planar Perpendicular Whipstitch with the Krackow Stitch And Standard Whipstitch Abstract Presentation 6 minutes Stefano Muscatelli, MD, FAOA, FAAOS, UNITED STATES Kempland C Walley, MD, UNITED STATES Conor Shea Locke, MS, UNITED STATES Joseph A Greenstein, BS, UNITED STATES Aaron David Sciascia, PhD, ATC, PES, SMTC, UNITED STATES David P Patterson, MD, UNITED STATES Michael T. Freehill, MD, UNITED STATES
Osteoarthritis Therapies in the Clinical Trial Pipeline: A 2020 Review Abstract Presentation 6 minutes Nicholas N. DePhillipo, PhD, MBA, ATC, UNITED STATES Zachary S. Aman, BA, UNITED STATES Travis Dekker, MD, UNITED STATES Gilbert Moatshe, MD, PhD, NORWAY Jorge Chahla, MD, PhD, UNITED STATES Robert F. LaPrade, MD, PhD, UNITED STATES
Effects of Blood Flow Restriction Therapy for Muscular Strength, Hypertrophy and Endurance in Healthy and Special Populations: a Systematic Review and Meta-Analysis Abstract Presentation 5 minutes Edward Perera, BSc, MBBS, UNITED KINGDOM Ming Zhu, MD Cand., UNITED KINGDOM Nolan Horner, MD, CANADA Asheesh Bedi, MD, UNITED STATES Olufemi R. Ayeni, MD, PhD, MSc, FRCSC, CANADA Moin Khan, MD, MSc, FRCSC, CANADA
Assessment of inter-rater reliability of a new device to measure anterior knee laxity. A cadaver study ePoster Presentation Alessandra Scaini, MD, ITALY Maristella Francesca Saccomanno, MD, PhD, ITALY Alessandro Colosio, MD, ITALY Marco Adriani, MD, ITALY Marcello Motta, MD, ITALY Mac Donald Tedah Djemetio, MD, ITALY Emanuele Maggini, MD, ITALY Giuseppe Bertoni, MD, ITALY Martina Re, MD, ITALY Giuseppe Frizziero, MD, ITALY Giuseppe Milano, Prof., ITALY
How Do We Evaluate Return to Sport After Anterior Cruciate Ligament Reconstruction? A Systematic Review Of Methodologies. Abstract Presentation 6 minutes Niv Marom, MD, ISRAEL William Xiang, BS, UNITED STATES Isabel Wolfe, BA, UNITED STATES Bridget Jivanelli, MLIS, UNITED STATES Riley J. Williams, MD, UNITED STATES Robert G. Marx, MD, UNITED STATES
Do Isolated Greater Tuberosity Fractures Behave Like Rotator Cuff Tears When Fixed Arthroscopically? A Matched Cohort Analysis Abstract Presentation 6 minutes Joseph J. Ruzbarsky, MD, UNITED STATES Dylan R. Rakowski, BS, UNITED STATES Thomas Woolson, BS, UNITED STATES Marilee P. Horan, MPH, UNITED STATES Peter J. Millett, MD, MSc, UNITED STATES
Acl Reconstruction Patients Have Increased Risk Of Knee Arthroplasty: Data From The Norwegian Knee Ligament Register And The Norwegian Arthroplasty Register From 2004-2019 Abstract Presentation 1 minutes Havard Visnes, MD, PT, PhD, NORWAY Tone Gifstad, MD, PhD, NORWAY Andreas Persson, MD, PhD, NORWAY Stein Håkon Låstad Lygre, PhD, NORWAY Lars Engebretsen, MD, PhD, NORWAY Jon Olav Drogset, Prof., NORWAY Ove Furnes, NORWAY
Burnout Self-Report Amongst Latinamerican Orthopedic Surgeons ePoster Presentation Alex Vaisman, MD, Prof., CHILE Rodrigo Guiloff, MD, Prof, CHILE Martin Contreras, MD, CHILE Francisco Figueroa, MD, CHILE David H. Figueroa, MD, CHILE Iris Delgado, CHILE Rafael Calvo, MD, CHILE
4DCT Analysis Of Cadaveric Knees To Determine Isometric Graft Positions in ACL Reconstruction – A Novel Technique Abstract Presentation 5 minutes Matthias Lu, MBBS (Hons) (Monash), AUSTRALIA Bronwyn Anderson, MBBS, BMedSci(Hons), MSpMed, AUSTRALIA Justin Wong, BMedSci, MBBS, FRACS(Orth), FAOrthA, AUSTRALIA
Evaluation Of Rotator Cuff Repair With And Without Concomitant Biceps Treatment: A Retrospective Review Of Patient Outcomes Abstract Presentation 5 minutes Daniel Nemirov, BA, UNITED STATES Zachary J Herman, MD, UNITED STATES Ryan W. Paul, BS, UNITED STATES Ari Clements, BA, UNITED STATES Matthew Beucherie, BS, UNITED STATES Christopher J. Hadley, BS, UNITED STATES Michael G. Ciccotti, MD, UNITED STATES Kevin Freedman, MD, UNITED STATES Brandon Erickson, MD, UNITED STATES Sommer Hammoud, MD, UNITED STATES Meghan E. Bishop, MD, UNITED STATES
Tranexamic Acid Administration In Arthroscopic Surgery Is A Safe Adjunct To Decrease Postoperative Pain And Swelling: A Systematic Review & Meta-Analysis Abstract Presentation 5 minutes Conor Jones, BSc HK, BHSc PA, MD(2022), CANADA Kyle Goldstein, BSc., CANADA Jeffrey Kay, MD, CANADA Jason J. Shin, MD, CANADA Darren L. de SA, MD MBA FRCSC, CANADA
Level Of Confidence Of Randomized Controlled Trials In Arthroscopy And Sports Medicine – A Spin Based Assessment Abstract Presentation 7 minutes Girinivasan Chellamuthu, MBBS, MS Ortho, FIOT, FASM, INDIA Sathish Muthu, MS Ortho., DNB Ortho., MNAMS., , INDIA
Does Suture Tape Reinforcement Lead To Improved 2-Year Clinical Outcomes For Posterior Cruciate Ligament Reconstruction? Abstract Presentation 5 minutes Erik Therrien, MD, MSc, FRCSC, CANADA Ayoosh Pareek, MD, UNITED STATES Bryant M. Song, MS, UNITED STATES Ryan R Wilbur, BS, UNITED STATES Michael J. Stuart, MD, UNITED STATES Bruce A. Levy, MD, UNITED STATES
Validity Of A Novel Sensor-Based Application For Home-Based Rehabilitation Following Anterior Cruciate Ligament Reconstruction Abstract Presentation 6 minutes Natalie Mengis, MD, SWITZERLAND Jules-Nikolaus Rippke, Dr. med. (MD), SWITZERLAND Sebastian Schmidt, GERMANY Mahli Zecher, M.Sc., GERMANY Caroline Schmidt-Lucke, MD, PhD, GERMANY Andree Ellermann, MD, GERMANY Christian Sobau, MD, GERMANY
Injury Risk to IIHF Ice Hockey Referees and Linesmen Abstract Presentation 6 minutes Charles A. Popkin, MD, UNITED STATES Ajay S Padaki, MD, UNITED STATES Andrew Rogers, UNITED STATES David P Trofa, MD, UNITED STATES T. Sean Lynch, MD, UNITED STATES Markku Tuominen , MD, PhD, FINLAND Michael J. Stuart, MD, UNITED STATES
High Specialization Among Female Youth Soccer Players Is Associated with an Increased Likelihood of Serious Injury: A Cross-Sectional Study Of Youth, College, and Professional Soccer Athletes Abstract Presentation 5 minutes Michelle Xiao, BS, UNITED STATES Jacie L Lemos, BS, UNITED STATES Calvin E Hwang, MD, UNITED STATES Seth L. Sherman, MD, UNITED STATES Marc R. Safran, MD, Prof., UNITED STATES Geoffrey D. Abrams, MD, UNITED STATES
Correlation Of PROMIS® Global Health Scores With Pediatric Activity Level Abstract Presentation 6 minutes Henry B. Ellis, MD, UNITED STATES Connor Carpenter, BBA, UNITED STATES Savannah Cooper, MS, UNITED STATES Benjamin Johnson, PA-C, UNITED STATES Charles W Wyatt, CPNP, UNITED STATES Shane Miller, MD, UNITED STATES Kevin G. Shea, MD, UNITED STATES Philip L. Wilson, MD, UNITED STATES
Peri-Operative Opioid Delivery After Paediatric Knee Arthroscopic Surgery ePoster Presentation Lee Benaroch, MD, BSc (Hons), CANADA James Meterissian, BA, CANADA Sami Chergui, MD, CANADA Jessica Collins, OTR/L, CANADA Sabrina Carrié, MD. FRCPC, CANADA Thierry Pauyo, MD, FRCSC, CANADA
Modified Suture Bridge Demonstrates Biomechanical Advantages For Tibial Eminence Fracture Fixation Abstract Presentation 5 minutes Nyaluma N. Wagala, MD, UNITED STATES Philipp Wilhelm Winkler, MD, Assoc. Prof., AUSTRIA Christopher M. Gibbs, MD, UNITED STATES Calvin K. Chan, MS, UNITED STATES Yulia A. Yatsenko, , UNITED STATES Thomas Rauer, SWITZERLAND Jonathan D Hughes, MD, PhD, UNITED STATES Richard E Debski, PhD, UNITED STATES Volker Musahl, MD, Prof., UNITED STATES
Patient Satisfaction And Functional Outcomes Of Multiple Joint Replacements: A Survey Study Of Patients Who Have Undergone Total Shoulder, Total Hip, And Total Knee Abstract Presentation 6 minutes Joseph Michael Brutico, BS, UNITED STATES Justin E Palm, BS, UNITED STATES Lasya Rangavajjula, BS, UNITED STATES Somnath Rao, BS, UNITED STATES Steven B. Cohen, MD, UNITED STATES James X Liu, MD, UNITED STATES
Comparison Of Outcomes Of High Tibial Osteotomy Using Two Principles Of Pre-Operative Planning Abstract Presentation 6 minutes Nisarg Paresh Shah, MS, Mch, UNITED KINGDOM Rafael Sales Fernández, MD, UNITED KINGDOM Kevin Syam, MBBS, MS, DNB ,Mch (T&O), FEBOT, FRCSEd(T&O), UNITED KINGDOM Benjamin Coupe, FRCS, UNITED KINGDOM Sijith Sasi, MS, Mch, UNITED KINGDOM
Assesment Of The Anterolateral Ligament By Magnetic Resonance Imaging Is A Predictor For Failure In The Anterior Cruciate Ligament Reconstruction Abstract Presentation 6 minutes David H. Figueroa, MD, CHILE Maria Loreto Figueroa Berrios, MD, CHILE Rodrigo Guiloff, MD, Prof, CHILE Nicolas Zilleruelo, MD, CHILE Francisco Figueroa, MD, CHILE Alex Vaisman, MD, Prof., CHILE
Identification of Predictive Risk Factors for the Development of a Stress Fracture within 6 Months in Female Elite Long-distance Runners Award Winner 5 minutes Tomoya Ishida, PhD, JAPAN Harukazu Tohyama, MD, PhD, JAPAN
Males and Females Have Different Walking Muscle Activity Patterns After ACL Injury and Reconstruction Abstract Presentation 7 minutes Elanna Arhos, PT, DPT, UNITED STATES Stephanie Di Stasi, PhD, PT, UNITED STATES Erin Heather Hartigan, PT, PhD, ATC, OCS, UNITED STATES Lynn Snyder-Mackler, PT, ScD, FAPTA, UNITED STATES
Comparison of the Single Assessment Numeric Evaluation (SANE) Score to the Short Version of the International Hip Outcome Tool (iHOT-12) for Assessment of Hip Conditions Abstract Presentation 6 minutes Brandon Barnds, MD, UNITED STATES Atlee Witt, BA, UNITED STATES Alexandra Orahovats, MS, UNITED STATES Kenneth J. Hunt, MD, UNITED STATES James W. Genuario, MD, UNITED STATES Theodore F. Schlegel, UNITED STATES
Bone Bruise Pattern And Mechanism Of Anterior Cruciate Ligament (ACL) Injury In Professional Footballers: Correlation Between MRI And Video-Analysis Abstract Presentation 5 minutes Pieter D'Hooghe, MD PhD MBA, QATAR Alberto Grassi, PhD, ITALY Francesco Della Villa, MD, ITALY Khalid Alkhelaifi, MD, QATAR Emmanouil Papakostas, MD, FEBSM, QATAR Stefano Zaffagnini, MD, Prof., ITALY Raouf Rekik, MD, PhD, QATAR
Can Talented Youth Soccer Players Who Have Undergone Anterior Cruciate Ligament Reconstruction Reach the Elite Level? Abstract Presentation 5 minutes Alexander Sandon, MD, SWEDEN Tor Söderström, Prof, SWEDEN Andreas Stenling, PhD, SWEDEN Magnus Forssblad, SWEDEN
Increased Tibiofemoral Rotation Angle Might Be Associated With Failure After Anterior Cruciate Ligament Reconstruction: A Matched-Control Pilot Study Abstract Presentation 4 minutes Chilan Leite, MD, PhD, UNITED STATES Gergo B Merkely, MD, PhD, UNITED STATES Molly Zgoda, MS, UNITED STATES Emily Hunt, PhD, UNITED STATES Julie Burland, PhD, UNITED STATES Christian Lattermann, MD, UNITED STATES
4-Domain Prom Tailored For Orthopedic Sports Medicine Abstract Presentation 5 minutes Sergio Rocha Piedade, MD, MSc, PhD, Associate Professor, BRAZIL Mark R. Hutchinson, Professor, MD, FACSM, FAANA, FAAOS, UNITED STATES Daniel Miranda Ferreira, Professor, BRAZIL Mario Ferretti, MD, PhD, BRAZIL Nicola Maffulli, MD, PhD, MS, FRCS(Orth), UNITED KINGDOM
3D Biomechanics Of Football Kick - Prediction Of Performance And Concurrent Injury Risk In Elite Footballers Abstract Presentation 5 minutes Janani Gopalakrishnan, MD (Sports Medicine), INDIA Vikram Rao, MD , INDIA Thiagarajan Alwar, MD, INDIA Suresh Perumal, MS(Orth), INDIA Prakash Ayyadurai, MS, INDIA Parthiban Jeganathan, MS, INDIA Karthik Anand Parachur, MS(Orthopaedics), INDIA Arumugam Sivaraman, MS(Orth), AB(IM)(USA), FRCS(Glasg), INDIA
Achieving Successful Outcomes In High-Level Athletes With Borderline Hip Dysplasia Undergoing Hip Arthroscopy With Capsular Plication And Labral Preservation: A Propensity Matched Controlled Study Abstract Presentation 4 minutes Andrew Jimenez, MD, UNITED STATES Peter Monahan, BS, UNITED STATES Kara Miecznikowski, BS, UNITED STATES Benjamin Saks, MD, UNITED STATES Hari Krishna Ankem, MD, UNITED STATES Payam William Sabetian, MD, GUATEMALA Ajay C. Lall, MD, MS, FAAOS, UNITED STATES Benjamin G. Domb, MD, UNITED STATES
Generalized Joint Hypermobility More Common in Surgical Failure Cases after Patellofemoral Stabilization Abstract Presentation 5 minutes Laurie A. Hiemstra, MD, PhD, FRCSC, CANADA Mark R Lafave, PhD, CANADA Sarah Kerslake, BPhty, MSc, CANADA
The Higher Rate of Injury Following the COVID-19 Lockdown Period in Elite Professional Footballers Highlights the Importance of Pre-Season Training Abstract Presentation 4 minutes Albert Tang, MBChB, BSc (Hon), MRCS, MSc SEM (Hon), PGDip SEM, UNITED KINGDOM John Ranson, MBChB, BSc (Hons), MRCSEd, UNITED KINGDOM Neil Jain, BM, MRCS(Ed), FRCS(Tr&Orth), UNITED KINGDOM
Biologically Augmented Suture for Ligament Bracing Positively Effects Human Ligamentocytes and Osteoblasts in vitro ePoster Presentation Alexander Otto, MD, GERMANY Mary Beth McCarthy, BA, UNITED STATES Joshua B. Baldino, PharmD, UNITED STATES Julian Mehl, MD, GERMANY Lukas Nawid Muench, MD, GERMANY Robert A. Arciero, MD, UNITED STATES Augustus D. Mazzocca, MS, MD, UNITED STATES
Evaluation Of Medial Patellofemoral Ligament Reconstruction In Inmature Skeleton. A Comparative Study Between Two Techniques Abstract Presentation 5 minutes Juan Miguel Del Castillo, MD, URUGUAY Martín Sierra, MD, URUGUAY Juan Dupont, URUGUAY Johan Von Heideken, MD, PhD, SWEDEN Juan Enrique Kenny Pujadas, MD, URUGUAY
Comparison Of Different Fixation Techniques Of The Long Head Of The Biceps Tendon (Lhbt) In Superior Capsule Reconstruction For Irreparable Posterosuperior Rotator Cuff Tears: A Dynamic Biomechanical Evaluation Abstract Presentation 9 minutes Daniel P. Berthold, Association.-Prof., GERMANY Lukas Nawid Muench, MD, GERMANY Felix Dyrna, MD, GERMANY Bastian Scheiderer, MD, GERMANY ELIFHO OBOPILWE, ME, BSc, UNITED STATES Michael R. Krifter, MD, AUSTRIA Giuseppe Milano, Prof., ITALY Ryan M Bell, BS, UNITED STATES Andreas Voss, MD, GERMANY Andreas B. Imhoff, MD, Prof. Emeritus, GERMANY Augustus D. Mazzocca, MS, MD, UNITED STATES Knut Beitzel, Prof. Dr., GERMANY
Long Term Clinical Outcome Of Combined Autologous Bone And Articular Cartilage Chip Transplantation For Osteochondral Lesions In The Knee Abstract Presentation 5 minutes Bjørn Borsøe Christensen, MD, PhD, DENMARK Morten Lykke Olesen, MD, PhD, DENMARK Casper B. Foldager, MD, PhD, DENMARK Kris T. C. Hede, MD, DENMARK Jonas Jensen, MD, PhD, DENMARK Martin Lind, MD, PhD, Prof., DENMARK
Lower Extremity Injury Rates On Artificial Turf And Natural Grass Playing Surfaces: A Systematic Review Abstract Presentation 6 minutes Heath Patrick Gould, MD, UNITED STATES Stephen J Lostetter, , UNITED STATES Eric R Samuelson, M.S., UNITED STATES Gregory P Guyton, MD, UNITED STATES

Menu

Search Filters

Nanowarming And Ice-Free Cryopreservation Of Large Articular Cartilage Specimens

Nanowarming And Ice-Free Cryopreservation Of Large Articular Cartilage Specimens

Introduction

Methods

Results

Discussion And Conclusion

More 2021 Congress Sports Medicine Abstract Presentations

More ISAKOS 2021: Global Content

Menu

Search Filters

Sign-in to ISAKOS

Nanowarming And Ice-Free Cryopreservation Of Large Articular Cartilage Specimens

Nanowarming And Ice-Free Cryopreservation Of Large Articular Cartilage Specimens

Introduction

Methods

Results

Discussion And Conclusion

More ISAKOS 2021: Global Content